Перегляд за Автор "Antonenko, S.P."

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Isolation, cultivation, quantification of cellular carotenoids and identification of cell wall sporopollenin in Neochloris dissecta (Korshikov) Tsarenko (Chlorococcum dissectum Korshikov) (Chlorophyta)
(Київ : Гідроекологічне товариство України, 2019-11-07) Strebko, N.P.; Oleksienko, A.A.; Antonenko, S.P.; Komaristaya, V.P.; Комариста, Вікторія Павлівна
The alga species investigated might be regarded as the potential source of carotenoids, possibly astaxanthin. The alga possesses a relatively high culture growth rate. Further research is necessary on exact species taxonomic attribution, factors of carotenoid accumulation induction, cell carotenoid content HPLC confirmation, and effective methods of carotenoid extraction from the cells with the cell walls containing sporopollenin.
Nutrient stored inside Dunaliella salina cells prevents beta-carotene accumulation under its medium deficiency
(Kherson State University, 2015-05-19) Komaristaya, V.P.; Antonenko, S.P.; Rudas, A.N.
At alternating cultivation cycles under deficiencies of nitrogen or phosphorus D. salina culture grew limitedly at the first cultivation, then intense after transferring the culture. Cellular beta-carotene accumulated during the first cultivation, and then almost did not. D. salina cells are able to accumulate the available nutrient at the other nutrient deficiency and to spend the intracellular nutrient reserve for culture growth when the other nutrient re-supplied to the medium.
Response of Dunaliella salina Teod. culture to various regimes of fed-batch nutrient supplementation
(Харківський національний аграрний університет, 2016) Komaristaya, V.P.; Antonenko, S.P.; Rudas, A.N.
There were studied D. salina culture growth, beta-carotene and total protein accumulation under various fed-batch regimes: supplementation of nitrate and phosphate, nitrate or phosphate solely, and after the switch of nitrate supplied culture to phosphate supplementation and vice versa. Pre-cultures, unsupplied with the both or any nutrient, accumulated beta-carotene. The sub-cultures supplied in turns with nitrogen and phosphorus, thus surviving one or the other nutrient depletion, regained culture growth ability, lost the ability to accumulate beta-carotene and started to accumulate protein. The ability to reserve the available nutrient under another nutrient deficiency was hypothesized as D. salina possible ecological strategy, including assimilated carbon storage in triacylglycerides (TAGs) and beta-carotene accumulation to prevent TAGs oxidation. The outcomes for industrial D. salina culture are discussed.
Variability of cultural and morphological traits of Dunaliella salina Teod. from different habitats
(V.N. Karazin Kharkov National University, 2013-12) Pasiuga, O.S.; Antonenko, S.P.; Komaristaya, V.P.; Rudas, A.N.
Deficiency of nitrogen and phosphorus induced beta-carotene accumulation in the cells of 35 clonal cultures of D. salina isolated from 6 habitats in Ukraine and 1 in Russia. Nitrate and phosphate re-supplied into the medium decreased cellular beta-carotene content and cell size. Repeated passage into the medium without nutrients recovered inducible beta-carotene level; the dimensional characteristics of the cells recovered partly. Isolates from different habitats (except one) broke up into 2 groups: low carotene – small celled – fast growing and high carotene – large celled – slow growing. Peculiarities of clonal cultures depended on habitats, where they were isolated from, and persisted for 3 passages, i.e. inherited. Each 5 isolates from the same habitat did not differ, i.e. D. salina populations were homogenous within a single habitat, at least at the time and location of sampling. The ability to beta-carotene accumulation induced by the deficit of nutrients can be extrapolated to D. salina species on the whole, at least to the populations originated from Ukraine and lake Baskunchak in Russia.