Наукові роботи. Факультет радіофізики, біомедичної електроніки та комп’ютерних систем
Постійне посилання колекціїhttps://ekhnuir.karazin.ua/handle/123456789/1138
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Документ Fluorimetric study of interaction between europium coordination complexes and DNA(Харьковский Национальный Университет им. В.Н. Каразина, 2009) Kutsenko, O.K.; Trusova, V.M.; Gorbenko, G.P.; Limanskaya, L.A.; Deligeorgiev, T.; Vasilev, A.; Kaloyanova, S.; Lesev, N.Lanthanide coordination complexes have found numerous applications in a number of areas, including laser techniques, fluorescent analysis, biomedical assays. Likewise, they exhibit antitumor properties. Eu(III) tris-β-diketonato complexes (EC) are newly synthesized compounds with high anticancer activity. Despite extensive studies, the detailed mechanism of their biological effects is far from being resolved. Examining the interactions between EC and biological molecules in model systems is essential for deeper understanding of the mechanisms behind their biological activity. In the present work we employed fluorescent probe acridine orange (AO) to investigate EC-DNA interaction. AO-DNA binding was followed by the marked fluorescence increase detected at 530 nm. EC addition suppressed this fluorescent changes. EC were found to differ in their ability to modify AO-DNA interactions. EC4 and EC6 have demonstrated the most pronounced effect on AO-DNA binding. AO-DNA complexation occurs predominantly via intercalation mode. EC are large planar structures, whose DNA intercalating ability was reported to increase with the planarity of ligands. It seems likely that AO and EC can compete for the binding sites on DNA molecule.Документ Comparative analysis of molecular mechanisms of relaxation of DNA bending deformation(Харьковский Национальный Университет им. В.Н.Каразина, 2010) Shashel, Ya.V.; Smyrnova, D.O.Comparison of relaxation mechanisms of bending DNA deformation was performed by molecular dynamics method for two types of bending DNA from complex with 434 repressor and purine repressor. Comparative analysis of relaxation dynamics of local structure parameters characterizing DNA structure on dinucleotide level and parameters characterizing DNA structure as whole was performed. Obtained data shows that relaxation takes place in stages and each stage is characterized by similar time intervals for two bending DNA types. It is shown that character of relaxation in the initial stages of dynamics depends on the type of DNA bending deformation: for smooth curve linear changes in parameters are peculiar; for localized sharp curve changes in parameters occurs abruptly.