Mechanism of activation by CA2+ of hemolysis induced by lytic polypeptide equinatoxin II
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2011
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Харьковский Национальный Университет им. В.Н.Каразина
Анотація
Equinatoxin II is a cytolytic polypeptide from the sea anemone Actinia equina L. which forms pores in natural and artificial membranes. In the present study, we show that the mechanism by which Ca2+ activates toxin-induced hemolysis of human red blood cells consists of the ability of Ca2+ which enters the cell presumably through preformed toxin-induced pores and acts inside the cell to increase the rate of toxin binding to the membrane. As a result, a larger amount of pores is produced thus increasing the rate of hemolysis. In addition, the data reveal that equinatoxin II induces hemolysis of RBC interacting with a limited number of toxin-binding sites (receptors) with an upper estimate of (180 35) 103 sites per one cell. The total number of toxin-binding sites does not depend on the presence of Ca2+. These data strongly suggest that specific receptor must exist on the erythrocyte membrane to mediate the hemolytic action of this toxin.
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erythrocyte, hemolysis, divalent cations, equinatoxin II
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