Наукові роботи. Біологічний факультет
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Документ Response of Dunaliella salina Teod. culture to various regimes of fed-batch nutrient supplementation(Харківський національний аграрний університет, 2016) Komaristaya, V.P.; Antonenko, S.P.; Rudas, A.N.There were studied D. salina culture growth, beta-carotene and total protein accumulation under various fed-batch regimes: supplementation of nitrate and phosphate, nitrate or phosphate solely, and after the switch of nitrate supplied culture to phosphate supplementation and vice versa. Pre-cultures, unsupplied with the both or any nutrient, accumulated beta-carotene. The sub-cultures supplied in turns with nitrogen and phosphorus, thus surviving one or the other nutrient depletion, regained culture growth ability, lost the ability to accumulate beta-carotene and started to accumulate protein. The ability to reserve the available nutrient under another nutrient deficiency was hypothesized as D. salina possible ecological strategy, including assimilated carbon storage in triacylglycerides (TAGs) and beta-carotene accumulation to prevent TAGs oxidation. The outcomes for industrial D. salina culture are discussed.Документ Nutrient stored inside Dunaliella salina cells prevents beta-carotene accumulation under its medium deficiency(Kherson State University, 2015-05-19) Komaristaya, V.P.; Antonenko, S.P.; Rudas, A.N.At alternating cultivation cycles under deficiencies of nitrogen or phosphorus D. salina culture grew limitedly at the first cultivation, then intense after transferring the culture. Cellular beta-carotene accumulated during the first cultivation, and then almost did not. D. salina cells are able to accumulate the available nutrient at the other nutrient deficiency and to spend the intracellular nutrient reserve for culture growth when the other nutrient re-supplied to the medium.Документ Variability of cultural and morphological traits of Dunaliella salina Teod. from different habitats(V.N. Karazin Kharkov National University, 2013-12) Pasiuga, O.S.; Antonenko, S.P.; Komaristaya, V.P.; Rudas, A.N.Deficiency of nitrogen and phosphorus induced beta-carotene accumulation in the cells of 35 clonal cultures of D. salina isolated from 6 habitats in Ukraine and 1 in Russia. Nitrate and phosphate re-supplied into the medium decreased cellular beta-carotene content and cell size. Repeated passage into the medium without nutrients recovered inducible beta-carotene level; the dimensional characteristics of the cells recovered partly. Isolates from different habitats (except one) broke up into 2 groups: low carotene – small celled – fast growing and high carotene – large celled – slow growing. Peculiarities of clonal cultures depended on habitats, where they were isolated from, and persisted for 3 passages, i.e. inherited. Each 5 isolates from the same habitat did not differ, i.e. D. salina populations were homogenous within a single habitat, at least at the time and location of sampling. The ability to beta-carotene accumulation induced by the deficit of nutrients can be extrapolated to D. salina species on the whole, at least to the populations originated from Ukraine and lake Baskunchak in Russia.Документ Ecological peculiarities of natural populations of hyperhalobe microalga Dunaliella salina Teod. in solar salt work ponds of the South of Ukraine and Russia(V.N. Karazin Kharkov National University, 2014-04) Komaristaya, V.P.; Rudas, A.A.; Tatischeva, N.M.; Tatischev, E.V.; Rudas, A.N.The paper presents the results of expedition research of some solar salt works of the South of Ukraine (Kherson region, AR Crimea) and lake Baskunchak (Astrachan region, Russia), as well as stationary observations on populations of the microalga Dunaliella salina Teod. in the ponds of Heroyske salt works (Gola Prystan’ district, Kherson region) that we carried out in 2005–2008. We discuss the approaches to modeling natural environment in D. salina laboratory culture to develop and optimize the process of industrial culturing in the open culture.Документ Effect size of some factors influencing productivity indexes in Dunaliella salina Teod. culture(Харьковский национальный университет им. В.Н. Каразина, 2011-02) Mil'ko, S.I.; Komaristaya, V.P.; Rudas, A.N.